【出版期刊】《International Journal of Biological Macromolecules》
【產品原文引用】
2.4. Immunization
Threehundred1-day-oldchicks were randomlydividedintofive groups (n = 60 per group). Groups I–V were injected subcutaneously with 0.2 ml of WO-OMP vaccine, PP-OMP vaccine, TPPPS-OMP vaccine, OMP-only vaccine and physiological saline, respectively, at 3,7 and 12 days old. The final concentrations of OMP were 500 g/ml at the first vaccination, 1 mg/ml at the second vaccination and 2 mg/ml at the third vaccination. Seven days after the third vaccination, 30 chicks from each group were challenged with 100 LD50 P. mirabilis strain Q1 by oral administration.
On days 3, 7, 14, 21, 28, 35, 42 and 49 after the first vaccination,the peripheral blood and duodenum were sampled for analysis.The antibody titers were detected using indirect enzyme-linked immunosorbent assay (ELISA), T-lymphocyte proliferation rates were detected with flow cytometry and secreting-type IgA (SIgA)and interleukin-2 (IL-2) were measured using ELISA kits (Shanghai Hengyuan Bio-technology Co. Ltd.). The pathogenesis and survival status of chicks were clinically examined daily for 14 successive days after challenge. The mortality, morbidity and protective rates in each group were calculated as follows:
